 |  Inter Milan - Fiorentina
Sunday November 29, 2009 @ Giuseppe Meazza |
 | Fiorentina - Atalanta
Sunday December 6, 2009 @ Stadio Artemio Franchi Florence |
 | Fiorentina - AC Milan
Sunday December 20, 2009 @ Stadio Artemio Franchi Florence |
 | Fiorentina - AS Bari
Sunday January 10, 2010 @ Stadio Artemio Franchi Florence |
| Fiorentina - Bologna
Sunday January 17, 2010 @ Stadio Artemio Franchi Florence |
 | Fiorentina - AS Roma
Sunday February 7, 2010 @ Stadio Artemio Franchi Florence |
| Fiorentina - Livorno
Sunday February 21, 2010 @ Stadio Artemio Franchi Florence |
 | Lazio Roma - Fiorentina
Sunday February 28, 2010 @ Olympic Stadium Rome |
 | Fiorentina - Juventus
Sunday March 7, 2010 @ Stadio Artemio Franchi Florence |
 | Napoli - Fiorentina
Sunday March 14, 2010 @ Stadio San Paolo |
| Fiorentina - Genoa
Sunday March 21, 2010 @ Stadio Artemio Franchi Florence |
 | Fiorentina - Udinese
Sunday March 28, 2010 @ Stadio Artemio Franchi Florence |
| Fiorentina - Inter Milan
Sunday April 11, 2010 @ Stadio Artemio Franchi Florence |
| AC Milan - Fiorentina
Sunday May 2, 2010 @ San Siro |
| Fiorentina - Siena
Sunday May 9, 2010 @ Stadio Artemio Franchi Florence |