 |  Wigan Athletic - Birmingham City
Saturday December 5, 2009 @ The JJB Stadium |
 | Stoke City - Wigan Athletic
Saturday December 12, 2009 @ Britannia Stadium |
 | Liverpool - Wigan Athletic
Wednesday December 16, 2009 @ Anfield Road |
 | Wigan Athletic - Bolton Wanderers
Saturday December 19, 2009 @ The JJB Stadium |
 | Wigan Athletic - Blackburn Rovers
Saturday December 26, 2009 @ The JJB Stadium |
 | Manchester United - Wigan Athletic
Monday December 28, 2009 @ Old Trafford |
 | Wigan Athletic - Aston Villa
Saturday January 9, 2010 @ The JJB Stadium |
| Wolverhampton Wanderers - Wigan Athletic
Saturday January 16, 2010 @ Molineux Stadium |
| Blackburn Rovers - Wigan Athletic
Wednesday January 27, 2010 @ Ewood Park |
 | Wigan Athletic - Everton
Saturday January 30, 2010 @ The JJB Stadium |
 | Sunderland - Wigan Athletic
Saturday February 6, 2010 @ Stadium of Light |
| Wigan Athletic - Stoke City
Tuesday February 9, 2010 @ The JJB Stadium |
 | Wigan Athletic - Tottenham Hotspur
Saturday February 20, 2010 @ The JJB Stadium |
| Birmingham City - Wigan Athletic
Saturday February 27, 2010 @ St Andrew's Ground |
| Wigan Athletic - Liverpool
Saturday March 6, 2010 @ The JJB Stadium |
| Bolton Wanderers - Wigan Athletic
Saturday March 13, 2010 @ Reebok Stadium |
| Wigan Athletic - Burnley
Saturday March 20, 2010 @ The JJB Stadium |
 | Manchester City - Wigan Athletic
Saturday March 27, 2010 @ City Of Manchester Stadium |
 | Fulham - Wigan Athletic
Saturday April 3, 2010 @ Craven Cottage |
 | Wigan Athletic - Portsmouth
Saturday April 10, 2010 @ The JJB Stadium |
 | Wigan Athletic - Arsenal
Saturday April 17, 2010 @ The JJB Stadium |
 | West Ham United - Wigan Athletic
Saturday April 24, 2010 @ Boleyn Ground |
 | Wigan Athletic - Hull City
Saturday May 1, 2010 @ The JJB Stadium |
 | Chelsea - Wigan Athletic
Sunday May 9, 2010 @ Stamford Bridge |